Muaj ntau yam xim uas siv rau hauv kev txiav cov khoom siv bioslicing, txhua yam muaj cov yam ntxwv thiab ntau yam kev siv sib txawv. Nov yog qee cov xim bioslicing uas siv ntau thiab lawv cov lus qhia luv luv:
Ua ntej, cov xim ntuj
Hematoxylin: Qhov no yog ib qho xim uas tau rho tawm los ntawm cov ceg qhuav ntawm South American hematoxylum (cov legume sov) los ntawm kev muab nws tso rau hauv ether. Hematoxylin tsis tuaj yeem zas xim ncaj qha, thiab nws yuav tsum tau oxidized kom dhau los ua oxyhematoxylin (tseem hu ua hematoxylin) ua ntej nws siv tau. Nws yog ib qho khoom siv zoo rau kev zas xim rau lub nucleus thiab tuaj yeem sib txawv cov qauv sib txawv hauv lub cell mus rau hauv ntau yam xim sib txawv.
Carmine: Carmine, tseem hu ua carmine lossis carmine, yog ua los ntawm cov kab cochineal poj niam sov sov uas qhuav thiab zom ua hmoov, rho tawm cov kab liab, thiab tom qab ntawd kho nrog alum los ua. Carmagenta kuj yog ib qho xim zoo rau lub nucleus, thiab cov qauv dyed tsis yooj yim ploj mus, tshwj xeeb tshaj yog rau tag nrho cov xim ntawm cov ntaub ntawv me me.
Qhov thib ob, cov xim dag
Acid fuchsin: Acid fuchsin yog cov xim acidic, hmoov liab, yaj hauv dej, yaj me ntsis hauv cawv. Nws yog tus neeg sawv cev zoo rau cov cell staining, siv dav hauv kev npaj tsiaj, hauv kev npaj cog rau daim tawv nqaij, pulp thiab lwm yam cell parenchyma thiab phab ntsa cellulose.
Liab Congo: Liab Congo yog ib qho xim zas uas muaj kua qaub, ua los ntawm hmoov liab jujube, yaj tau hauv dej thiab cawv, xiav hauv kua qaub. Nws feem ntau siv rau hauv kev tsim cov nroj tsuag ua ib txheej rau hematoxylin lossis lwm yam xim zas ntawm lub hlwb, thiab kuj tseem siv tau los pleev xim rau cytoplasm thiab cov hlab ntsha.
Xim ntsuab khov kho: Xim ntsuab khov kho yog xim acidic, yaj tau hauv dej thiab cawv. Nws yog ib hom xim rau cov ntaub so ntswg cellulose uas muaj plasma, uas siv dav hauv kev zas xim rau cov hlwb thiab cov ntaub so ntswg ntawm cov nroj tsuag.
Sudan III: Sudan III yog ib hom xim zas uas muaj kua qaub tsis muaj zog, hmoov liab, yaj tau hauv roj thiab cawv. Nws yog ib hom xim zas uas feem ntau siv los qhia cov roj hauv cov ntaub so ntswg.
Eosin: Muaj ntau hom eosin, thiab feem ntau siv eosin Y yog cov xim acidic, uas yog xim liab nrog cov siv lead ua xiav me me lossis hmoov xim av. Eosin siv dav hauv kev npaj tsiaj, yog cov xim cytoplasmic zoo, thiab feem ntau siv ua cov xim interlining rau hematoxylin.
Fuchsin yooj yim: fuchsin yooj yim yog cov xim alkaline, uas siv dav hauv kev tsim khoom siv roj ntsha thiab tuaj yeem siv los pleev xim rau cov collagen fibers thiab cov elastic fibers.
Crystal violet: Crystal violet yog ib qho alkaline zas xim, siv dav hauv cytology, histology thiab bacteriology, yog ib qho xim zoo, feem ntau siv rau kev staining nuclear.
Gentian violet: Gentian violet yog cov xim sib xyaw ua ke ntawm cov xim alkaline, feem ntau yog cov xim crystal violet thiab methyl violet, uas tuaj yeem siv hloov pauv nrog crystal violet thaum tsim nyog.
Cov xim no ua lub luag haujlwm tseem ceeb hauv kev ua kom cov plaub hau tsis zoo, thiab los ntawm ntau txoj kev sib txawv thiab kev sib xyaw ua ke, lawv tuaj yeem pom tseeb cov qauv morphological ntawm cov hlwb thiab cov ntaub so ntswg, muab kev txhawb nqa tseem ceeb rau kev tshawb fawb txog tsiaj txhu thiab kev kho mob.
Lub sijhawm tshaj tawm: Lub Yim Hli-08-2024